Course on SAXS  from Biological Material

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* Course on SAXS  from Biological Material*

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Date: October 4-5, 2011 Location: Advance Light Source (ALS) at Lawrence Berkeley National Laboratory , Berkeley, CA

The significance of Small Angle X-ray Scattering (SAXS) technique has been rediscovered in recent years by an increasing number of structural biologists to complement high resolution structural studies by crystallography, EM and NMR. SIBYLS team will host workshop with strong emphasis on experimental aspects of non-crystalline diffraction techniques in biology. The two-day workshop will provide training on experimental techniques and software tutorial sessions primarily for biological SAXS studies. One day workshop will be dedicated for data processing by workshop participants. The latest advances in SAXS studies on biological systems will be reported and discussed by several experts in a diverse spectrum of structural biology benefiting from non-crystalline diffraction studies (see program bellow). Also planned are presentations on complementary experimental approaches and modeling techniques. Participants will receive updates on current developments at SIBYLS and development in the software dedicated to analyze SAXS for structural biology.

Due to the limited space availability only first 40 registered participants will be accepted. 



Organizers: Michal Hammel, Greg Hura



Inquires: Jane Tanamachi JTanamachi@lbl.gov



Registration: To attend “Course on SAXS” you need register for the 2010 Advanced Light Source Users’ Meeting. ALS user meeting will be held at Berkeley Lab beginning Monday, October 3. “Practical Course on SAXS” will begin Thuesday October 4th and continue through Wednesday October 5. When you registering, you must indicate “Course on SAXS from Biological Materials” . Registration Fee: The early registration fee is $225.00 (regular) and $95.00 (student); after Friday, September 24, the registration fee will be $250.00 (regular) and $115.00 (student).

PROGRAM

Tuesday, October 4th LBNL (room 54-130-Pers. Hall)

12:45 Welcoming Remarks Michal Hammel

12:50 Robert Rambo, LBNL, Berkeley “Small-Angle Scattering and its Application to Soft matter Science: Historical Remarks” “New ways to analyze SAXS from biological material”

13:50 Greg Hura, LBNL, Berkeley
“SIBYLS SAXS capabilities and future developments

15:00 Coffee Break

15:15 Stefan Arold UCSF, The University of Texas, Housten “SAXS as a tool to study multi-domain proteins and protein multimerisation “

15:45 Alexei Kazantsev, UC Boulder, Colorado “RNase P RNA: from pieces of the crystal structure to the complete structure in solution. “

16:15 Alexander Johs, Oak Ridge National Laboratory, Oak Ridge “SAXS reveals the intramolecular metal ion transfer between flexibly-linked domains of mercuric ion reductase”

16:45 Jack Tanner, University of Missouri-Columbia, Columbia , “SAXS Studies of the Quaternary Structures of Proline Utilization A (PutA) Proteins”

Wednesday, October 5th LBNL (room 54-130-Pers. Hall),

Tutorial session,

9:00 Michal Hammel, LBNL, Berkeley “Criteria for evaluation of SAXS results”

9:30 Rob Rambo, LBNL, Berkeley “OPTIMUS: New software for SAXS data processing”

10:00 Dina Schneidman UCSF, San Francisco ” SAXS software in Integrative Modeling Platform (IMP) and Chimera Interface “

10:30 Coffee Break

10:45 Greg Hura, Robert Rambo and Michal Hammel, LBNL, Berkeley “Data reduction and processing tutorial”

12:00 Lunch Break

13:00-16:00 Participating Students “Practical Session - Data reduction and processing”

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