Recently in protocols Category

Burgeoning crystallographers may find the high-throughput screening (HTS) laboratory, which is part of the Center for High-Throughput Structural Biology (CHTSB) at the Hauptman-Woodward Institute (HWI) to be a very logical starting point for determining the suitability of a particular sample for macromolecular crystallography studies. The HWI will prepare crystal-growth screening experiments in 1536-well microassay plates for about $300 per sample. More details are available on their website and in the FAQ.

Quen Cheng in the Cooper lab has done a nice set of experiments to address the usefulness of various readily available detergents for improving the behavior proteins in SAXS experiments.

A new review on macromolecular SAXS has been published in the Quarterly Reviews in Biophysics by Putnam, C.D., Hammel, M., Hura, G.L., and Tainer, J.A.

"This six part review addresses both theoretical and practical concepts, concerns and considerations for using these techniques in conjunction with computational methods to productively combine solution scattering data with high-resolution structures."

I added an entry in the PX protocol section that should help more advanced general users to switch from SAXS to PX and then tune up the beam for PX use.