IDAT - Integrated Diffraction Analysis Technologies

DOE BER aims to overcome energy and environmental challenges by utilizing powerful microbial communities whose gene’s sequences and regulation are researched at a revolutionary pace through tremendous DOE investments. All cellular processes are regulated and coordinated by the dynamically changing multi-component complexes these genes encode. Structures of these macromolecular machines and functional complexes are central to rationally direct microbial communities for BER specific outcomes. To usefully complement the pace of BER’s genomic efforts with accurate and dynamic structural information of biologically relevant complexes and conformations, the Structurally Integrated Biology for Life Sciences (SIBYLS) beamline 12.3.1 and the University of California MRPI beamline 8.3.1 at the Advanced Light Source (ALS) have joined strengths in this Integrated Diffraction Analysis Technologies (IDAT) Program Proposal. IDAT will continue to be at the forefront of providing structural information from small angle X-ray scattering (SAXS) in high throughput and combining this information with detailed macromolecular crystallographic (MX) analyses with links to X-ray tomography (XT). Major IDAT objectives are to 1) develop, test, and provide productive facilities and technologies for characterizations of molecular machines, including integrated and independent synchrotron-based biophysical characterizations. 2) innovate new computational tools and metrics to facilitate experiment design, data analyses, and interpretation of SAXS plus MX and XT. 3) advance the limits of structural investigation for BER programs characterizing, predicting, and directing microbial pathways. Through innovative technologies, analysis tools, and collaborations, IDAT seeks to develop and provide order of magnitude enhancements in capabilities to translate structural data into useful results and interpretations for DOE BER missions.

IDAT Resources

Beamline 2.1 (National Center for X-ray Tomography)
Beamline 8.3.1 (TomAlberTron aka The University of California MRPI Beamline)
Beamline 12.3.1 (SIBYLS Beamline)

DOE-funded investigators who wish to initiate collaborations or want preferred access to IDAT resources are encouraged to contact IDAT Staff directly.

IDAT User Map