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Hello DOMO users,
DOMO is fairly robust, and is capable of handling your precious crystals mounted in a variety of bases:
However, you must take some care when gluing or epoxying the pins into the bases. If there is too much glue or epoxy or you inadvertantly get some on the sides or bottom of the base this will cause the robot to jam, which will require time-wasting reset procedures, lost samples, and unhappy beamline support personnel.
Here is a recent example of several pins where the user (who will remain unnamed) applied entirely too much epoxy. Somehow the user was able to load these pins into the cassette, but they caused the robot to jam.
There are more detailed tips and hints on the SSRL SMB website for preparing your bases and pins.
Burgeoning crystallographers may find the high-throughput screening (HTS) laboratory, which is part of the Center for High-Throughput Structural Biology (CHTSB) at the Hauptman-Woodward Institute (HWI) to be a very logical starting point for determining the suitability of a particular sample for macromolecular crystallography studies. The HWI will prepare crystal-growth screening experiments in 1536-well microassay plates for about $300 per sample. More details are available on their website and in the FAQ.
A new review on macromolecular SAXS has been published in the Quarterly Reviews in Biophysics by Putnam, C.D., Hammel, M., Hura, G.L., and Tainer, J.A.
“This six part review addresses both theoretical and practical concepts, concerns and considerations for using these techniques in conjunction with computational methods to productively combine solution scattering data with high-resolution structures.”
The review provides an extensive and up-to-date review on the application of small angle X-ray scattering is available for download.
I added an entry in the PX protocol section that should help more advanced general users to switch from SAXS to PX and then tune up the beam for PX use.