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Hello DOMO users,
DOMO is fairly robust, and is capable of handling your precious crystals mounted in a variety of bases:
However, you must take some care when gluing or epoxying the pins into the bases. If there is too much glue or epoxy or you inadvertantly get some on the sides or bottom of the base this will cause the robot to jam, which will require time-wasting reset procedures, lost samples, and unhappy beamline support personnel.
Here is a recent example of several pins where the user (who will remain unnamed) applied entirely too much epoxy. Somehow the user was able to load these pins into the cassette, but they caused the robot to jam.
There are more detailed tips and hints on the SSRL SMB website for preparing your bases and pins.
The crystals server (an Apache Tomcat webapp) has been installed. The crystals webapp will allow you to upload an excel spreadsheet, containing a list of samples, to the SIBYLS beamline for use with DOMO. The spreadsheet will be automatically converted to an XML file and will become available to you via Blu-Ice once you start data collection. The webapp was developed at the SSRL and is in use at their Macromolecular Crystallography facility. If you have used the SAM robot at Stanford and uploaded your spreadsheet to their server then you will notice a distinct similarity.
DOMO (Dynamic Offsite MX(Macromolecular Crystallography) Operator) mounted crystals remotely for the first time today. The user accessed the beamline via port 22 (i.e. ssh) from San Diego using NX Client and was able to successfully screen through 50 or so crystals. Although I could have monitored the shift from my office, from home, or from Tahiti, I thought it would be more prudent to remain close to the beamline in the unlikely event that intervention was needed…. Well I’m glad that I stuck around. Although DOMO performed well, there were a couple of times that I needed to step in a make things right. Overall, however, it was a very successful first run.
If you are interested in collecting crystallographic data from the comfort of your own lab (or Tahiti) I would encourage you to apply for time on the SIBYLS beamline. We have a small supply of sample cassettes and the necessary tools for loading the cassettes. A cassette kit can be sent to you, whereupon you load the cassette with your valuable samples, and thusly send it back to the beamline in time for your shift. It’s loads of fun!
Scott Williams, a postdoc in the Tainer group at The Scripps Research Institute, was the first crystallographer to use DOMO. Here he is kicking back and enjoying some of the fine espresso available at the beamline while DOMO mounts his samples from the custom LN2 sample dewar in the hutch.
Scott screened through ~40 samples during his 16 hour shift, and collected several useful datasets.
After determining that operating the LN2 sample dewar without a lid was going to be problematic with regards to ice accumulation in the liquid nitrogen I decided to design and install a pneumatically operated guillotine lid. Here is a nice aerial shot of the closed lid.
hit the jump for details and more pics…
Today DOMO mounted its first pin from the sample dewar that was filled with liquid nitrogen.
This video is pretty large so unless you are on a fast connection or have alot of time to waste trust me DOMO is finally crawling.