Fill plate wells with 24µL of sample and buffer.
Three sample concentrations between 1-10 mg/ml.
For redundancy, it is recommended to collect two (identical) buffers for each concentration series, one before and one after.
Do not use greater than 5% glycerol. It has been shown that 2% provides the maximum benefit. With glycerol, the viscosity of the solution increases and bubbles can become a problem. See Methods paper for more information.
Fill in your spreadsheet as follows:
ContainerID. "LabName". Write the name of your lab name here (no spaces).
ContainerType. "plate". Do not change this column.
Well. Indicates well location. Do not change any value in this column.
SampleID. Replace the well number with the name of the data output file (Sample ID). Only letters, numbers and underscores are accepted (no spaces).
Collect. When checked ("x"), the sample will be collected (in order, starting from the top). If there is no sample or buffer in the well, leave it blank.
Directory. Each sample will have it's own directory named after the well number. For example "A1", "A2", etc. If there is buffer in the well, add a "b" after the well number. This is very important for accurate data processing.
WashAfter. Indicates a wash step. When checked, the sample cell will be thoroughly rinsed three times after that well is exposed. It is recommended to wash after a concentration series.
|LabName||plate||B4||B4 (no sample)|
Once you have booked a mail in slot, a unique barcode will be created for your plate.
Print this label out and tape it to the side of your plate.
Please ship overnight to arrive at SIBYLS lab on the first day of shift:
Lawrence Berkeley Lab
1 Cyclotron Road
Berkeley, CA 94720
ATTN: Kathryn Burnett / SYBILS 12.3.1
1. Seal plate securely with recommended sealing mat. DO NOT use adhesive based sealing film. Be sure label is securely taped to side of plate.
2. Place sealed plate in plastic bag or wrap with parafilm.
3. Using two 4°C cold packs (NOT frozen), place one on top and one on bottom of plate.
4. Rubberband the assembly together for placement within styrofoam shipping box.
5. Tighty pack styrofoam box by surrounding assembly with more 4°C cold packs. Do not allow plate to touch any frozen packs. DO NOT ship with wet ice.
6. Clearly label the outside of your box with the temperature of your samples. This is extremely important if a shipping delay occurs.
7. Provide us with a tracking number.
We require all users to register with the ALS user office. You will not have access to your data until you are registered.
We also ask that users submit one ALS RAPIDD beamtime proposal (not General User proposal) for up to three mail-in uses.
This is for record keeping only, and will not affect your chances of using our service. So far, the high throughput capabilities of our endstation have allowed us to accept every user that has requested mail in SAXS time.
You will be notified by email that your samples have arrived.
The plate will be stored at the appropriate temperature: 4°C or -80°C.
It will be lightly spun at 3700 rpm for 10 minutes at 4°C prior to data collection.
It will be held at 10°C during data collection, which lasts aprox. 4 hours for a full plate.
You will be notified by email as soon as your data is available.
You will get back integrated scattering profiles in ASCI format in a results directory for each sample. A series of exposures, in equal sub-second time slices, are taken of each well.
In addition, you will receive a report with an initial assessment of the sample from our beamline staff.
Learn about SAXS collection at SIBYLS...README
Learn about common problems with SAXS samples...SAXS Analysis