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Mail In Instructions the basics

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Sample Format

Fill plate wells with 24µL of sample and buffer.

Three sample concentrations between 1-10 mg/ml.

For redundancy, it is recommended to collect two (identical) buffers for each concentration series, one before and one after.

Buffer Properties:

Do not use greater than 2% glycerol. It has been shown that 2% provides the maximum benefit. With glycerol, the viscosity of the solution increases and bubbles can become a problem. See Methods paper for more information.

96-Well Plate

    VWR Catalog Numbers

  • Full skirt 96-well plate10011-228
  • AxyMat Sealing Mat10011-130


Spreadsheet Once filled out, upload to SIBYLS SAXS database


Fill in your spreadsheet as follows:

  • Column A

    ContainerID. "LabName". Write the name of your lab name here (no spaces).

  • Column B

    ContainerType. "plate". Do not change this column.

  • Column C

    Well. Indicates well location. Do not change any value in this column.

  • Column D

    SampleID. Write the name for the data output file (Sample ID). Only letters, numbers and underscores are accepted (no spaces).

  • Column E

    Collect. When checked ("x"), the sample will be collected (in order, starting from the top). If there is no sample or buffer in the well, leave it blank.

  • Column F

    Directory. Each sample will have it's own directory named after the well number. For example "A1", "A2", etc. If there is buffer in the well, add a "b" after the well number. This is very important for accurate data processing.

  • Column G

    WashAfter. Indicates a wash step. When checked, the sample cell will be thoroughly rinsed three times after that well is exposed. It is recommended to wash after a concentration series.

instructions.html.erb
A B C D E F G
ContainerID ContainerType Well SampleID Collect Directory WashAfter
LabName plate A1 buffer1 x A1b
LabName plate A2 protein_1_low x A2
LabName plate A3 protein_1_medium x A3
LabName plate A4 protein_1_high x A4x
LabName plate A5 buffer1 x A5b
LabName plate A6 buffer2 x A6b
LabName plate A7 protein_2_low x A7
LabName plate A8 protein_2_medium x A8
LabName plate A9 protein_2_high x A9 x
LabName plate A10 buffer2 x A10b
LabName plate A11 buffer3 x A11b
LabName plate A12 protein_3_low x A12
LabName plate B1 protein_3_medium x B1
LabName plate B2 protein_3_high x B2 x
LabName plate B3 buffer3 x B3b
LabName plate B4 B4 (no sample)




Barcode_image_example

Once you have booked a mail in slot, a unique barcode will be created for your plate.

Print this label out and tape it to the side of your plate.

Shipping Information

Please ship overnight to arrive at SIBYLS lab on the first day of shift:

Lawrence Berkeley Lab
1 Cyclotron Road
MS 6R2136
Berkeley, CA 94720
ATTN: Kathryn Burnett / SYBILS 12.3.1
510-685-5755

How to Safely Pack Your Plate:

1. Seal plate securely with recommended sealing mat. DO NOT use adhesive based sealing film. Be sure label is securely taped to side of plate.

2. Place sealed plate in plastic bag or wrap with parafilm.

3. Using two 4°C cold packs (NOT frozen), place one on top and one on bottom of plate.

4. Rubberband the assembly together for placement within styrofoam shipping box.

5. Tighty pack styrofoam box by surrounding assembly with more cold packs. Okay if these are frozen, but do not allow plate to touch any frozen packs. DO NOT ship with wet ice.

6. Clearly label the outside of your shipping box with the temperature of your samples. This is extremely important if a shipping delay occurs.

7. Provide us with a tracking number.


ALS User Office

We require all users to register with the ALS user office. You will not have access to your data until you are registered.

Register Here


We also ask that users submit one ALS RAPIDD beamtime proposal (not General User proposal) for up to three mail-in uses.

This is for record keeping only, and will not affect your chances of using our service. So far, the high throughput capabilities of our endstation have allowed us to accept every user that has requested mail in SAXS time.


On Arrival what happens when your plate arrives

You will be notified by email that your samples have arrived.

The plate will be stored at the appropriate temperature: 4°C or -80°C.

It will be lightly spun at 3700 rpm for 10 minutes at 4°C prior to data collection.

It will be held at 10°C during data collection, which lasts aprox. 4 hours for a full plate.

You will be notified by email as soon as your data is available.


Data Package what you will get back

You will get back integrated scattering profiles in ASCI format in a results directory for each sample. A series of exposures, in equal sub-second time slices, are taken of each well.

In addition, you will receive a report with an initial assessment of the sample from our beamline staff.


Details, details if you want to know more

Learn about SAXS collection at SIBYLS...

README

Learn about common problems with SAXS samples...

SAXS Analysis