Month: November 2011

Flexibility and Mechanism of Ub-PCNA in DNA Repair

Tsutakawa *et al.* have combined solution Small Angle X-ray Scattering (SAXS) data collected at the SIBYLS beamline with computational modeling carried out at Oak Ridge Leadership Computing Facility ( OLCF ) to elucidate new modes of flexibility in a key protein complex (Ubiquitin-PCNA) involved in DNA replication and repair. The work was published in the…
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Insights into Architecture and Mechanism in the Autophagy Pathway

SAXS data collected at the SIBYLS beamline was used in conjunction with high resolution crystals structures to discern details of the unique interaction mode of of these key players in the autophagy pathway. > Atg7 is a noncanonical, homodimeric E1 enzyme that interacts with the noncanonical E2 enzyme, Atg3, to mediate conjugation of the ubiquitin-like…
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Tautomeric form of C•A mismatch is stabilized by polymerase.

Using data collected at the SIBYLS beamline members of the Beese Lab have demonstrated that high fidelity polymerases can stabilize certain tautomeric forms of mismatched base pairs ultimately resulting in incorporation of mutations into the DNA duplex. >Even though high-fidelity polymerases copy DNA with remarkable accuracy, some base-pair mismatches are incorporated at low frequency, leading…
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