Science Highlights

Using SAXS to evaluate radical scavengers

SIBYLS user Tim Stachowski and the Snell group make the cover of the Journal of Synchrotron Radiation for their paper describing their work using Small-angle X-ray Scattering to evaluate how three scavengers commonly used in crystallographic experiments perform in solution at 10°C. In this study, they found the SAXS-based method can detect damage at X-ray…
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SIBYLS makes cover of Protein Science

SIBYLS scientists Michal Hammel and John Tainer describe the mechanistic insights into NHEJ structural biochemistry determined by small angle X-ray scattering (SAXS) results combined with X-ray crystallography (MX) and cryo-electron microscopy (cryo-EM). The cover image, like the old car engine malfunctioning without a linchpin and flexible belt, shows the NHEJ efficiency arises from a flexible…
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SAXS study of radiation damage

SIBYLS user Dr. Tim Stachowski from the Snell group at Hauptman-Woodward Medical Research Institute,   led a research effort combining a protein engineering approach with SAXS to monitor cleavage of a specific bond from exposure to the X-ray beam. X-ray induced disulfide bond breakage is a common phenomenon in X-ray crystallography but there is limited information…
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Study finds ‘Missing Link’ in the Evolutionary History of Carbon-fixing Protein Rubisco

SIBYLS beamline scientists contribute to the discovery of an ancient form of rubisco, the most abundant enzyme on earth and critical to life as we know it. By analyzing SEC-SAXS data collected at 12.3.1, they were able to capture how the enzyme’s structure changes during different states of activity.      READ MORE

Scaffold protein compacts human DNA Ligase

SIBYLS scientist, Michal Hammel, analyzing data collected at the SIBYLS beamline gained insight into the shape and conformational flexibility of the XL as well as XRCC1 and DNA ligase IIIα (LigIIIα) alone. This ligase complex is critical for DNA single-strand break repair, a key target for PARP inhibitors in cancer cells deficient in homologous recombination.…
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Evolving SAXS Versatility

In this top 10 cited article using HT-SAXS data collected at SIBYLS, Chris Brosey, along with John Tainer, describe the value and versatility of Small Angle X-ray Scattering. The authors discuss evolving SAXS theory, methods, and applications that extend the field of small-angle scattering beyond simple shape characterization. From capturing architecture and dynamics to enabling…
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How Proteins Remodel DNA in Bacteria under Stress

By analyzing SAXS data collected at BL 12.3.1, SIBYLS scientists were able to determine the overall shapes of HU-DNA complexes in solution, helping to clarify how proteins remodel bacterial DNA in response to stressful environments. This discovery, enabled by three beamlines at the Advance Light Source, could lead to new strategies for controlling microbial behavior…
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Two recent papers from Doudna and Marletta groups combine SAXS with Cryo-EM

The Doudna group, explained in their recent paper,  “Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a” combine SAXS with Cryo-Em to show the C-terminal binding domain is sufficient for Cas12a inhibition. CRISPR-Cas systems provide bacteria and archaea with programmable immunity against mobile genetic elements. Evolutionary pressure by CRISPR-Cas has driven bacteriophage to evolve small protein…
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