Science Highlights

SIBYLS help signal target for Anticancer Drugs

Researchers from Genentech in collaboration with SIBYLS beamline scientist, Michal Hammel, used Small Angle X-Ray scattering to learn how an assembly of three proteins works together to transmit signals for cell division. The work reveals new targets for the development of drugs to fight certain types of cancer, including lung, colorectal, and pancreatic.


Sample Centering with Google’s AutoML

SIBYLS beamline scientist, Scott Classen, collaborated with ScienceIT consultants Shawfeng Dong and Fengchen Liu to use AutoML machine learning for the LoopDHS project. As part of the effort, IT student intern Jordan Jung developed a training dataset for the model to improve the accuracy. These developments enabled Dr. Classen to develop loopDHS a custom software…
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SIBYLS makes cover of Antibody Therapeutics

Analyzing SAXS data collected at SIBYLS beamline 12.3.1, Michal Hammel determined the structural arrangement of the VH and VL domains in the COBRA™ (COnditional Bispecific Redirected Activation) T-cell engagers. The study showed that the structural arrangement of the domains in COBRA is different from the expected yet the stable fold is maintained. Findings provide insights…
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Study finds ‘Missing Link’ in the Evolutionary History of Carbon-fixing Protein Rubisco

SIBYLS beamline scientists contribute to the discovery of an ancient form of rubisco, the most abundant enzyme on earth and critical to life as we know it. By analyzing SEC-SAXS data collected at 12.3.1, they were able to capture how the enzyme’s structure changes during different states of activity.      READ MORE

Using SAXS to evaluate radical scavengers

SIBYLS user Tim Stachowski and the Snell group made the cover of the Journal of Synchrotron Radiation for their paper describing their work using Small-angle X-ray Scattering to evaluate how three scavengers commonly used in crystallographic experiments perform in solution at 10°C. In this study, they found the SAXS-based method can detect damage at X-ray…
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SIBYLS makes cover of Protein Science

SIBYLS scientists Michal Hammel and John Tainer describe the mechanistic insights into NHEJ structural biochemistry determined by small angle X-ray scattering (SAXS) results combined with X-ray crystallography (MX) and cryo-electron microscopy (cryo-EM). The cover image, like the old car engine malfunctioning without a linchpin and flexible belt, shows the NHEJ efficiency arises from a flexible…
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SAXS study of radiation damage

SIBYLS user Dr. Tim Stachowski from the Snell group at Hauptman-Woodward Medical Research Institute, led a research effort combining a protein engineering approach with SAXS to monitor cleavage of a specific bond from exposure to the X-ray beam. X-ray induced disulfide bond breakage is a common phenomenon in X-ray crystallography but there is limited information…
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Scaffold protein compacts human DNA Ligase

SIBYLS scientist, Michal Hammel, analyzing data collected at the SIBYLS beamline gained insight into the shape and conformational flexibility of the XL as well as XRCC1 and DNA ligase IIIα (LigIIIα) alone. This ligase complex is critical for DNA single-strand break repair, a key target for PARP inhibitors in cancer cells deficient in homologous recombination.…
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