In this paper, published in Nucleic Acid Research, the authors used small-angle X-ray scattering (SAXS) combined with advanced computational approaches to characterize the conformational variability and DNA-binding properties of PNK. Extensive use of the SAXS capabilities of the SIBYLS beamline allowed the authors to visualize a flexible attachment of the FHA domain to the catalytic…
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In this paper, published online today in Nature Methods, the high-throughput SAXS capabilities of the SIBYLS beamline are demonstrated quite nicely. We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data…
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For anyone writing grants this analysis by Rands ought to ring true. It is geared towards the business world, but clearly has relevance to the scientific sphere.
In a recent article in the General Physiology and Biophysics we describe an analysis tool using relatively inexpensive small angle X-ray scattering (SAXS) measurements to identify protein flexibility and validate a constructed minimal ensemble of models, which represent highly populated conformations in solution. The resolution of these results is sufficient to address the questions being…
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Julie Tubbs from John Tainer’s group at Scripps published a totally sweet paper in the June 11, 2009 issue of Nature demonstrating that the ATL protein uses nucleotide flipping to link alkylated base damage to the nucleotide excision repair pathway. This publication was in part made possible by the SIBYLS beamline. Abstract: Alkyltransferase-like proteins (ATLs) share…
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For older browsers, or non-flash enabled mobile browsers, we offer the old skool status page which has convenient small png files showing the last 24 hours of beamline data, but if you want to have a hands-on experience you might want to try out our new Google-style annotated time lines. If you’re familiar with the Google finance…
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The structure of XPD was solved using data collected at the SIBYLS beamline and published in Cell this past summer. Recently this outstanding work was featured in the ALS Science Highlights. “XPD helicase is an enzyme that unwinds the DNA double helix; it is one component of an essential repair mechanism that maintains the integrity of…
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Stephen Quake on disclosure and peer review in scientific publications. Peer review is the bedrock value of the scientific community and although it certainly is not perfect, it is, to paraphrase Winston Churchill, “the worst system, except for all the others that have been tried.”