By analyzing SAXS data collected at BL 12.3.1, SIBYLS scientists were able to determine the overall shapes of HU-DNA complexes in solution, helping to clarify how proteins remodel bacterial DNA in response to stressful environments. This discovery, enabled by three beamlines at the Advance Light Source, could lead to new strategies for controlling microbial behavior…
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SIBYLS has recently installed a new Agilent 1290 Infinity II LC system to enhance our SEC-SAXS capabilities with a high reliability and robustness, improving analytical, instrument, and laboratory efficiency. Features include more chromatographic resolution, higher peak capacity for challenging separations, lower carryover for uncompromised data quality, unique detection capabilities, faster injection cycles with dual needle…
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Save the date! SIBYLS will be holding a SAXS workshop during the ALS User meeting from August 25-27, 2020. Details about the workshop coming soon. You can find general information about the User Meeting on the ALS website.
The Doudna group, explained in their recent paper, “Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a” combine SAXS with Cryo-Em to show the C-terminal binding domain is sufficient for Cas12a inhibition. CRISPR-Cas systems provide bacteria and archaea with programmable immunity against mobile genetic elements. Evolutionary pressure by CRISPR-Cas has driven bacteriophage to evolve small protein…
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SIBYLS is pleased to announce SAXS Frameslice, a new Webapp for merging frame sliced SAXS data. The Webapp was designed by Dave Shin with the purpose of improving your SAXS calculations by averaging the intensities of different regions of your data from multiple images of the same sample. With this Webapp you can: –…
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Central challenges in the design of large and dynamic macromolecular assemblies for synthetic biology lie in developing effective methods for testing design strategies and their outcomes, including comprehensive assessments of solution behavior. The authors of this paper created and validated an advanced design of a 600-kDa protein homododecamer that self-assembles into a symmetric tetrahedral cage.…
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Date: October 2 – 3, 2019 Location: Building 33 at Lawrence Berkeley National Laboratory, Berkeley, CA Building / ROOM : 33-306 This two-day workshop will cover frontiers in biological SAXS and will provide participants with software tutorial sessions for biological SAXS in addition to hands-on training in experimental techniques. The latest advances in SAXS studies…
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** Date: October 3 – 4, 2018 Location: Advanced Light Source at Lawrence Berkeley National Laboratory, Berkeley, CA Building 33 room 306 The two-day workshop, held during [ALS user meeting](https://als.lbl.gov/user-meeting/ “ALS user meeting”), will provide participants with software tutorial sessions for biological SAXS in addition to hands-on training in experimental techniques. The latest advances in…
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