DNA double strand break repair via nonhomologous end joining is a critical regulatory function that maintains genomic integrity. One of the major factors involved in this process is the XLF-XRCC4 protein complex. Although mutation of either XLF or XRCC4 leads to defects in break repair, the function of the XLF-XRCC4 complex has remained enigmatic. In…
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Special guest speaker, Dina Schneidman from the Sali lab presented a SAXS analysis webinar for members of the NCI program project – Structural Biology of DNA repair (SBDR) on July 14, 2011. She presented how to use the FOXS/FOXS-MES server and an example application. She introduced FOXSDOCK (which is almost ready). Additionally, she showed how…
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** Course on SAXS from Biological Material** Date: October 4-5, 2011 Location: Advance Light Source (ALS) at Lawrence Berkeley National Laboratory , Berkeley, CA The significance of Small Angle X-ray Scattering (SAXS) technique has been rediscovered in recent years by an increasing number of structural biologists to complement high resolution structural studies by crystallography, EM…
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We have been operating our SAXS mail-in/hand-in program for 6 months – 3 cycles of ALS proposals. The beamline and its staff are dedicated to developing and applying technologies and methodologies which combine SAXS and crystallography. Interacting with an extensive and robust external user community is a critical component of this development. Our mission for…
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Unstructured proteins, RNA or DNA components provide functionally important flexibility that is key to many macromolecular assemblies throughout cell biology. As objective, quantitative experimental measures of flexibility and disorder in solution are limited, small angle scattering (SAS), and in particular small angle X-ray scattering (SAXS), provides a critical technology to assess macromolecular flexibility as well…
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Two related manuscripts appear in the April 15 issue of CELL reporting results endo and exo nucleases important for DNA repair. Both groups of researchers made extensive use of the MX capabilities of the SIBYLS beamline to collect critical crystallographic data. The crystal structures of human flap endonuclease (FEN1) in complex with both substrate and…
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Rad50 is an ABC-type ATPase that forms a complex with the nuclease Mre11 and plays an essential role in the signaling and repair of DNA damage. Now crystal structures and SAXS analyses determined here at the SIBYLS beamline, along with functional assays, reveal how Rad50 transmits information between the ATPase and Mre11-binding sites, and the…
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From the January edition of “[Crystallography Times](http://www.rigaku.com/newsletter.html)” from Rigaku: > Small Angle X-ray Scattering (eds. Glatter & Kratky) can be freely (and legally) [downloaded](http://physchem.kfunigraz.ac.at/sm/Service/Glatter_Kratky_SAXS_1982.zip) from the [Scattering Methods](http://physchem.kfunigraz.ac.at/sm/) program at the University of Graz. The book provides a basic introduction to SAXS, followed by detailed fundamental theoretical and experimental aspects, as well as methods for…
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