Summary of Options for Applying for Beamtime at the ALS 1. [RAPIDD](http://www-als.lbl.gov/index.php/component/content/article/701-rapidd-proposal-process.html) – a rapid access process, replaces the 2-month proposal system. SAXS proposals should use the RAPIDD system. MX applicants may apply for either RAPIDD or 6 Month Proposals. The aim is to provide quick turnaround. Proposals are fairly simple, requiring a one page…
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3rd annual SIBYLS bioSAXS workshop is ON !! Date: October 9-10, 2012 Location: Advance Light Source (ALS) at Lawrence Berkeley National Laboratory , Berkeley, CA The SIBYLS team will host a workshop with strong emphasis on experimental aspects of Small Angle X-ray Scattering techniques in structural biology. The two-day workshop will provide training on experimental…
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We bring to your attention a nice review published recently in the European Biophysics Journal describing the theoretical and practical considerations when using SAXS to characterize macromolecular flexibility. >The dynamics of macromolecular conformations are critical to the action of cellular networks. Solution X-ray scattering studies, in combination with macromolecular X-ray crystallography (MX) and nuclear magnetic…
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SAXS was used to characterize the structural effects of phosphorylation events that modulate the ability of retinoblastoma protein to associate with E2F and other proteins. This work has been published in Genes & Development in the May 8th Advanced Online Articles section. >Cyclin-dependent kinase (Cdk) phosphorylation of the Retinoblastoma protein (Rb) drives cell proliferation through…
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Poly ADP-ribosylation regulates cellular processes such as genomic stability maintenance, transcription and cell death. The structure of rat poly(ADP-ribose) glycohydrolase has been determined using crystallographic data collected at the SIBYLS beamline, giving insights into the enzyme’s endoglycosidase activity and providing a basis for the development of therapeutic inhibitors. > Reversible post-translational modification by poly(ADP-ribose) (PAR)…
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Researchers with JCSG have recently determined the structure of [TadZ](http://www.pdb.org/pdb/explore/explore.do?structureId=3fkq) bound to ATP. This new PDB along with the structures of other pilins and pilin associated proteins have been chosen as the [PSI featured system of the month](http://sbkb.org/kb/archives.jsp?pageshow=49) for April. Bacteria typically have a large number of genes that encode proteins for the synthesis, localization…
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Researchers from [Scripps Research Institute](http://www.scripps.edu/news/press/20120209getzoff.html) and the University of Glasgow have published a detailed molecular model of how UVR8, a unique heme-free plant photoreceptor, senses UV-B light via an intricate interacting mesh of Tryptophan residues positioned at the UVR8 dimer interface which ultimately results in the disruption of a salt bridge and dissociation of the…
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Members of the Noller lab recently published a 3.3 Å resolution structure of a complex containing the ribosomal release factor (RF3) locked in its GTP-bound state (mimicked using GDPNP) in association with the *E. coli* 70s ribosome. Crystallographic data for this project was collected at the SIBYLS beamline MX station. > The class II release…
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