Members of the Noller lab recently published a 3.3 Å resolution structure of a complex containing the ribosomal release factor (RF3) locked in its GTP-bound state (mimicked using GDPNP) in association with the *E. coli* 70s ribosome. Crystallographic data for this project was collected at the SIBYLS beamline MX station. > The class II release…
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Tsutakawa *et al.* have combined solution Small Angle X-ray Scattering (SAXS) data collected at the SIBYLS beamline with computational modeling carried out at Oak Ridge Leadership Computing Facility ( OLCF ) to elucidate new modes of flexibility in a key protein complex (Ubiquitin-PCNA) involved in DNA replication and repair. The work was published in the…
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SAXS data collected at the SIBYLS beamline was used in conjunction with high resolution crystals structures to discern details of the unique interaction mode of of these key players in the autophagy pathway. > Atg7 is a noncanonical, homodimeric E1 enzyme that interacts with the noncanonical E2 enzyme, Atg3, to mediate conjugation of the ubiquitin-like…
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Using data collected at the SIBYLS beamline members of the Beese Lab have demonstrated that high fidelity polymerases can stabilize certain tautomeric forms of mismatched base pairs ultimately resulting in incorporation of mutations into the DNA duplex. >Even though high-fidelity polymerases copy DNA with remarkable accuracy, some base-pair mismatches are incorporated at low frequency, leading…
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DNA double strand break repair via nonhomologous end joining is a critical regulatory function that maintains genomic integrity. One of the major factors involved in this process is the XLF-XRCC4 protein complex. Although mutation of either XLF or XRCC4 leads to defects in break repair, the function of the XLF-XRCC4 complex has remained enigmatic. In…
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Special guest speaker, Dina Schneidman from the Sali lab presented a SAXS analysis webinar for members of the NCI program project – Structural Biology of DNA repair (SBDR) on July 14, 2011. She presented how to use the FOXS/FOXS-MES server and an example application. She introduced FOXSDOCK (which is almost ready). Additionally, she showed how…
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** Course on SAXS from Biological Material** Date: October 4-5, 2011 Location: Advance Light Source (ALS) at Lawrence Berkeley National Laboratory , Berkeley, CA The significance of Small Angle X-ray Scattering (SAXS) technique has been rediscovered in recent years by an increasing number of structural biologists to complement high resolution structural studies by crystallography, EM…
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We have been operating our SAXS mail-in/hand-in program for 6 months – 3 cycles of ALS proposals. The beamline and its staff are dedicated to developing and applying technologies and methodologies which combine SAXS and crystallography. Interacting with an extensive and robust external user community is a critical component of this development. Our mission for…
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