SIBYLS user Dr. Tim Stachowski from the Snell group at Hauptman-Woodward Medical Research Institute, led a research effort combining a protein engineering approach with SAXS to monitor cleavage of a specific bond from exposure to the X-ray beam. X-ray induced disulfide bond breakage is a common phenomenon in X-ray crystallography but there is limited information…
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SIBYLS scientist, Michal Hammel, analyzing data collected at the SIBYLS beamline gained insight into the shape and conformational flexibility of the XL as well as XRCC1 and DNA ligase IIIα (LigIIIα) alone. This ligase complex is critical for DNA single-strand break repair, a key target for PARP inhibitors in cancer cells deficient in homologous recombination.…
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In this top 10 cited article using HT-SAXS data collected at SIBYLS, Chris Brosey, along with John Tainer, describe the value and versatility of Small Angle X-ray Scattering. The authors discuss evolving SAXS theory, methods, and applications that extend the field of small-angle scattering beyond simple shape characterization. From capturing architecture and dynamics to enabling…
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By analyzing SAXS data collected at BL 12.3.1, SIBYLS scientists were able to determine the overall shapes of HU-DNA complexes in solution, helping to clarify how proteins remodel bacterial DNA in response to stressful environments. This discovery, enabled by three beamlines at the Advance Light Source, could lead to new strategies for controlling microbial behavior…
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SIBYLS has recently installed a new Agilent 1290 Infinity II LC system to enhance our SEC-SAXS capabilities with a high reliability and robustness, improving analytical, instrument, and laboratory efficiency. Features include more chromatographic resolution, higher peak capacity for challenging separations, lower carryover for uncompromised data quality, unique detection capabilities, faster injection cycles with dual needle…
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Save the date! SIBYLS will be holding a SAXS workshop during the ALS User meeting from August 25-27, 2020. Details about the workshop coming soon. You can find general information about the User Meeting on the ALS website.
The Doudna group, explained in their recent paper, “Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a” combine SAXS with Cryo-Em to show the C-terminal binding domain is sufficient for Cas12a inhibition. CRISPR-Cas systems provide bacteria and archaea with programmable immunity against mobile genetic elements. Evolutionary pressure by CRISPR-Cas has driven bacteriophage to evolve small protein…
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SIBYLS is pleased to announce SAXS Frameslice, a new Webapp for merging frame sliced SAXS data. The Webapp was designed by Dave Shin with the purpose of improving your SAXS calculations by averaging the intensities of different regions of your data from multiple images of the same sample. With this Webapp you can: –…
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