Our 6th annual SIBYLS bioSAXS workshop “Frontiers in biological SAXS” was a huge success. A big thank you to all of you who attended and a special thanks to our fabulous speakers: – Robert Rambo, Principal Beamline Scientist for the solution state SAXS beamline B21 at the Diamond Light Source, Oxfordshire, UK – John J.…
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The facilities and staff at the SIBYLS beamline contributed to this recent structural study of a human antibody that is able to neutralize Marburg and Ebola viruses. >The filoviruses, including Marburg and Ebola, express a single glycoprotein on their surface, termed GP, which is responsible for attachment and entry of target cells. Filovirus GPs differ…
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SAXS and MX data collected at SIBYLS enabled insights into protein flexibility in one of the proteins involved in Lou Gerigh’s disease. The results were recently published in PNAS and have been highlighted by [Today At Berkeley Lab](http://newscenter.lbl.gov/2014/10/13/berkeley-lab-and-scripps-research-institute-scientists-link-als-progression-to-increased-protein-instability/) and and [Daily Cal](http://www.dailycal.org/2014/10/16/berkeley-lab-scripps-institute-propose-cause-als/). >Protein framework alterations in heritable Cu, Zn superoxide dismutase (SOD) mutants cause misassembly…
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For posterity.
Recently in Science: >Structure determination of gold nanoparticles (AuNPs) is necessary for understanding their physical and chemical properties, but only one AuNP larger than 1 nanometer in diameter [a 102-gold atom NP (Au102NP)] has been solved to atomic resolution. Whereas the Au102NP structure was determined by x-ray crystallography, other large AuNPs have proved refractory to…
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We are pleased to announce the 5th annual SIBYLS bioSAXS workshop “Frontiers in biological SAXS” Date: October 7-8, 2014 Location: Advance Light Source (ALS) at Lawrence Berkeley National Laboratory , Berkeley, CA Small angle scattering (SAS) is experiencing a dramatic increase in popularity within the structural biology community. The availability of synchrotron radiation, low-noise detectors,…
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A new publication is available that describes practical aspects of collecting High-throughput SAXS data at the SIBYLS beamline, with a focus on challenging low concentration samples. Additional practical advice is laid out with respect to interpreting the resulting data. Dyer KN, Hammel M, Rambo RP, Tsutakawa SE, Rodic I, Classen S, Tainer JA, Hura GL.…
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The SIBYLS beamline was instrumental in providing key structural data for two recent publications exploring the dynamic nature of DNA repair. >The Mre11‐Rad50 complex is highly conserved, yet the mechanisms by which Rad50 ATP‐driven states regulate the sensing, processing and signaling of DNA double‐strand breaks are largely unknown. Here we design structure‐based mutations in Pyrococcus…
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